![]() ![]() In order to address the subtle differences in the spectra, we calculated different correlation coefficients, namely the Pearson coefficient and the Normalized cross correlation coefficient. All of the spectra are dominated by the magnetic bead substrate and no obvious differences between the sample types are apparent. For each sample type, eight independent replicates were considered. A series of Raman spectra were measured on three types of samples, namely SARS-CoV-2, Influenza A H1N1 virus and a negative control. The proposed approach is also applicable for other virus species when the specific recognition element is exchanged. The subsequent Raman measurements directly enable discriminating SARS-CoV-2-positive and -negative samples. The beads were functionalized with the angiotensin-converting enzyme 2 (ACE2) receptor protein, which is used as a recognition element to selectively enrich SARS-CoV-2 on the surface of the magnetic beads. We introduce a magnetic bead-based sample preparation scheme for enabling the Raman spectroscopic differentiation of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2)-positive and -negative samples.
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